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vitro transcription  (TaKaRa)


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    TaKaRa vitro transcription
    Vitro Transcription, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1083 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+transcripts/T7+RNA+Polymerase/bio_rxiv__64898__2026__05__12__724569-74-21-27
    Average 96 stars, based on 1083 article reviews
    vitro transcription - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Generated:

    Article Title: Ribozyme-Mediated Inhibition of Caspase-3 Protects Cerebellar Granule Cells from Apoptosis Induced by Serum–Potassium Deprivation
    Article Snippet: .. RNA transcripts were generated using T7 RNA polymerase (Ambion, Austin, TX) and purified by gel filtration chromatography (Clontech, Cambridge, UK). ..

    Purification:

    Article Title: Ribozyme-Mediated Inhibition of Caspase-3 Protects Cerebellar Granule Cells from Apoptosis Induced by Serum–Potassium Deprivation
    Article Snippet: .. RNA transcripts were generated using T7 RNA polymerase (Ambion, Austin, TX) and purified by gel filtration chromatography (Clontech, Cambridge, UK). ..

    Filtration:

    Article Title: Ribozyme-Mediated Inhibition of Caspase-3 Protects Cerebellar Granule Cells from Apoptosis Induced by Serum–Potassium Deprivation
    Article Snippet: .. RNA transcripts were generated using T7 RNA polymerase (Ambion, Austin, TX) and purified by gel filtration chromatography (Clontech, Cambridge, UK). ..

    Chromatography:

    Article Title: Ribozyme-Mediated Inhibition of Caspase-3 Protects Cerebellar Granule Cells from Apoptosis Induced by Serum–Potassium Deprivation
    Article Snippet: .. RNA transcripts were generated using T7 RNA polymerase (Ambion, Austin, TX) and purified by gel filtration chromatography (Clontech, Cambridge, UK). ..



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    Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of <t>RNA‐LNPs</t> by dynamic light scattering. (c) qRT‐PCR quantification of the <t>transcription</t> efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.
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    Image Search Results


    Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of RNA‐LNPs by dynamic light scattering. (c) qRT‐PCR quantification of the transcription efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.

    Journal: Transboundary and Emerging Diseases

    Article Title: A Self‐Amplifying RNA Lipid Nanoparticle (saRNA‐LNP) Vaccine Provides Effective Protection Against Porcine Epidemic Diarrhea

    doi: 10.1155/tbed/3115893

    Figure Lengend Snippet: Production and characteristics of PEDV‐S mRNA and saRNA vaccines. (a) PEDV‐S saRNA vaccine design schematic. (b) Average particle size and PDI of RNA‐LNPs by dynamic light scattering. (c) qRT‐PCR quantification of the transcription efficiency in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (d) Western blotting analysis PEDV‐S protein expression in 293T cells transfected with saRNA‐ or mRNA‐LNPs at indicated time points. (e) Densitometric quantification of PEDV‐S expression relative to β‐tubulin based on the blots of (d). Results in panels (c) and (e) were shown as mean ± SD of three independent experiments.

    Article Snippet: The transcription reaction consisted of linearized template DNA, N1‐methylpseudouridine‐5 ′ ‐triphosphate (Novoprotein, China), CAP GAU m7G(5 ′ )ppp(5")(2 ′ 0MeA)pU (SYNTHGENE, China), and components from the T7 High‐Yield RNA Transcription kit (Novoprotein, China).

    Techniques: Vaccines, Quantitative RT-PCR, Transfection, Western Blot, Expressing